An oligonucleotide with morpholine rings replacing backbone elements of standard DNA/RNA, binds stably to complementary RNA sequences.
Chromatin immunoprecipitation sequencing
RNA immunoprecipitation experiment assayed by sequencing.
A chromatin immunoprecipitation experiment assayed by microarray.
RNA immunoprecipitation experiment assayed by microarray.
Assay for transposase-accessible chromatin using sequencing (ATAC-Seq), is a method based on direct in vitro transposition of sequencing adaptors into native chromatin, and is a rapid and sensitive method for integrative epigenomic analysis. ATAC-Seq captures open chromatin sites using a simple two-step protocol.
Assay for transposase-accessible chromatin sequencing
biotinylated CxxC affinity purification sequencing
An assay in which DNA is the input molecule derived from an antibody based selection process using antibodies targeting methylated DNA, which is then sequenced using high throughput sequencing technology.
methylated DNA immunoprecipitation sequencing
Hi-C is a method for analysing 3-dimensional organisation and interaction of chromatin, belonging to the class of techniques collectively called "Chromosome Conformation Capture", in which chromatin is crosslinked, then digested, and re-ligated in such a way that only DNA fragments that are covalently linked together form ligation products. Together with 5C, Hi-C does not limit to the study to a specific locus in the genome, and can be used to study chromatin interaction across the genome. To achieve high-throughput, a biotin-labeled nucleotide is incorporated at the ligation junction, enabling selective purification of chimeric DNA ligation junctions followed by deep sequencing. Mapping the sequenced reads back to genome will reveal the origin of the fragments involved in the ligation events.
Tethered Chromosome Conformation Capture
Injection of a single sperm into a single celled embryo or an egg for the purpose of in vitro fertilization.
Whole Transcriptome Termini Site Sequencing.
Whole Transcriptome Termini Site Sequencing
N,N-dimethylethylenediamine (DMED) phosphoramidate-modified antisense oligonucleotide injection.
Locked Nucleic Acid antisense oligonucleotide injection.
A method of gene targetting transgenesis, both transisent and heritable, using the bacterial CRISPR system with specificity from guide RNA sequences.
clustered regularly interspaced short palindromic repeats-associated system.
CLIP is an antibody-based technique used to study RNA-protein interactions related to RNA immunoprecipitation (RIP), but differs from RIP in the use of UV radiation to cross-link RNA binding proteins to the RNA that they are bound to.
UV Cross-Linking and Immunoprecipitation
A chromatin immunoprecipitation assay targeting epigenetic modifications.
A chromatin immunoprecipitation assay targeting transcription factors.
Immunoprecipitation assay on otherwise unclassified targets.
A morpholino which is intended as an experimental control.
A morpholino targeting a splice site within an mRNA.
A morpholino targeting the translational start site of an mRNA, blocking translation and protein production.
A morpholino that has not been characterized.
A morpholino that contains a photosensitive cleavage site.
The emission of energy as electromagnetic waves or as moving subatomic particles, especially high-energy particles which cause ionization.
X-radiation, a form of electromagnetic radiation.
A method of gene targetting transgenesis, both transisent and heritable, using modified Fokl endonuclease domain recognition domains coupled to bacterial TAL effector system domains for specificity.
transcription activator-like effector nucleases
Data about protein function suitable for GO curation.
Data relevant to human diseases are contained within the article.
Data relevant to the copyright classification of an article for curation.
Open Access copyright permissions classification for an article.
No Access copyright permissions classification for an article.
Full Access: Request Permission copyright classification for an article.
Full Access: Permission Requested copyright classification for an article.
Full Access: Permission Granted copyright classification for an article.
Full Access: Permission Denied copyright classification for an article.
Creative Commons Attribution-NoDerivatives license
Open Access: CC BY-ND (Attribution, no derivatives)
Creative Commons Attribution-NonCommercial license
Open Access: CC BY-NC (Attribution, noncommercial)
Creative Commons Attribution-NonCommercial-NoDerivatives license
Open Access: CC BY-NC-ND (Attribution, noncommercial, no derivatives)
Creative Commons Attribution-NonCommercial-ShareAlike license
Open Access: CC BY-NC-SA (Attribution, noncommercial, share-alike)
Creative Commons Attribution-ShareAlike license
Open Access: CC BY-SA (Attribution, share-alike)
A method which assesses transcript expression by reverse transcription of purified mRNA into the corresponding complementary DNA (cDNA) before insertion into a vector, allowing synthesis of a large number of copies of the sequence for analysis.
cDNA clone-based transcript expression assay
A sequence of 3-6 nucleotides, typically of the form NGG, immediately adjacent to a guide RNA target site. This site is required for cleavage to occur in the CRISPR gene editing system.
A genotype is a variant genome, complete or incomplete.
A variation in the nucleic acid sequence of a specific gene.
A morpholino with a delivery moiety promoting uptake through the cell membrane.
Whole Transcriptome Start Site Sequencing.
Whole Transcriptome Start Site Sequencing
Dissociation of an embryo, tissue, or explant by chemical, enzymatic or mechanical methods into indivdual cells.
Reaggregation of an embryo, tissue, or explant from indivdual cells.
A morpholino targeting the microRNA response element in a gene's 3'UTR protecting the gene from miRNA mediated repression.
A technique for imaging surfaces and measuring various electrical or mechanical properties.